Our laboratory studies the cellular and molecular mechanisms through which toxic environmental agents cause lung diseases, especially pulmonary fibrosis and asthma. Using a variety of molecular techniques, genomics and transgenic mouse models we have identified intracellular signaling pathways and novel genes that contribute to the progression of environmental lung diseases.
The emerging field of nanotechnology has prompted new initiatives aimed at understanding the possible health effects of engineered nanomaterials (carbon nanotubes and nano-sized metal catalysts). Our goal is to explore and elucidate mechanisms of disease initiation and progression caused by engineered nanoparticles. We also seek to identify physical and chemical properties of nanomaterials that trigger fibrotic or allergic reactions in the lung in order to provide information for the design of safer nanomaterials. Of particular interest is determining the pathogenic potential of nanomaterials in susceptible populations and individuals with pre-existing disease, especially asthma. Our research, funded by The National Insitute of Environmental Health Sciences (NIEHS), is integrated with a national Nanotechnology Health Implications Consortium (NCNHIR) comprised of NIEHS-funded investigators to collectively understand the health risks associated with nanotechnology. Our research provides fundamental information for determining the potential human health risks of emerging nanotechnologies, which will be essential for the design of safe nanotechnologies in the future.
Research Areas: lung fibrosis, asthma, nanotoxicology, metals, particles, fibers.
Visit Bonner Lab Webpage for more information
For more information on nanotechnology research at NCSU, see: “Nano at NCSU”
See New Book Released Summer of 2012: “The Toxicology of Carbon Nanotubes” From Cambridge University Press and Amazon.
Selected Peer-Reviewed Publications
Glista EE, Taylor AJ, Sayers BC, Thompson EA, Bonner JC. Nickel nanoparticles synergistically enhance PDGF-induced expression of chemokines by rat pleural mesothelial cells via prolonged activation of MAP kinase signaling. Am. J. Respir. Cell Mol. Biol. 2012, June 14th [Epub ahead of print]
Lee JK, Sayers BC, Chun K-S, Lao H-C, Bonner JC, Langenbach. Multi-walled carbon nanotubes induce COX-2 expression via a MAP kinase-dependent mechanism in cultured RAW264.7 macrophages. Part. Fibre Toxicol. 2012, 9(1): 14.
Bonner JC. Carbon nanotubes as delivery systems for respiratory disease: do the dangers outweigh the potential benefits? Expert Rev Respir Med. 2011 Dec;5(6):779-87.
Wang X, Xia T, Addo Ntim S, Ji Z, Lin S, Meng H, Chung CH, George S, Zhang H, Wang M, Li N, Yang Y, Castranova V, Mitra S, Bonner JC, Nel AE. Dispersal State of Multiwalled Carbon Nanotubes Elicits Profibrogenic Cellular Responses That Correlate with Fibrogenesis Biomarkers and Fibrosis in the Murine Lung. ACS Nano. 2011 Nov 22. [Epub ahead of print]
Mossman BT, Lippmann M, Hesterberg TW, Kelsey KT, Barchowsky A, Bonner JC. Pulmonary endpoints (lung carcinomas and asbestosis) following inhalation exposure to asbestos. J Toxicol Environ Health B Crit Rev. 2011;14(1-4):76-121.
Bonner, J.C. (2010) Mesenchymal cell survival in airway and interstitial lung fibrosis. Fibrogenesis and Tissue Repair. Aug 25;3(1):15.
Bonner, J.C. (2010) Nanoparticles as a potential cause of pleural and interstitial lung disease. Proc Am Thorac Soc. 7(2):138-41.
Cesta, M.F., J.P. Ryman-Rasmussen, D.G. Wallace, T. Masinde, G. Hurlburt, A.J. Taylor, and J.C. Bonner. (2010) Bacterial Lipopolysaccharide Enhances PDGF Signaling and Pulmonary Fibrosis in Rats Exposed to Carbon Nanotubes. Am J Respir Cell Mol Biol. 43(2): 142-151.
Turpin, E.A., A. Antao-Menezes, M.F. Cesta, J.B. Mangum D.G. Wallace, E. Bermudez, and J.C. Bonner. (2010) Respiratory syncytial virus infection reduces lung inflammation and fibrosis in mice exposed to vanadium pentoxide. Respir. Res. 11:20.
Ryman-Rasmussen, J.P., M.F. Cesta, A.R. Brody, J.K. Shipley-Phillips, J.I. Everitt, E.W. Tewksbury, O.R. Moss, B.A. Wong, D.E. Dodd, M.E. Andersen, and J.C. Bonner. (2009) Inhaled carbon nanotubes reach the subpleural tissue in mice. Nature Nanotechnol. Nov;4(11):747-51. Epub 2009 Oct 25.
Ryman-Rasmussen, J.P., E.W. Tewksbury, O.R. Moss, M.F. Cesta, B.A. Wong, and J.C. Bonner. (2009) Inhaled multiwalled carbon nanotubes potentiate airway fibrosis in a murine model of allergic asthma. Am J Respir Cell Mol Biol.40: 349-358.
Card, J.W., D.C. Zeldin, J.C. Bonner, and E.R. Nestmann. (2008) Pulmonary applications and toxicity of engineered nanoparticles. Am J Physiol Lung Cell Mol Physiol. 2008 Sep;295(3):L400-11.
Antao-Menezes, A., E.A. Turpin, P.C. Bost, J.P. Ryman-Rasmussen, and J.C. Bonner . (2008) STAT-1 signaling in human lung fibroblasts is induced by vanadium pentoxide through an IFN-beta autocrine loop. J. Immunol. 180(6):4200-4207.
Voltz, J.W., J.W. Card, M.A. Carey, L.M. Degraff, C.D. Ferguson, G.P. Flake, J.C. Bonner , K.S. Korach, and D.C. Zeldin. (2008) Male Sex Hormones Exacerbate Lung Function Impairment After Bleomycin-Induced Pulmonary Fibrosis. Am. J. Respir. Cell. Mol. Biol . [Epub ahead of print].
Bonner, J.C. (2007) Lung fibrotic responses to particle exposure. Toxicol Pathol . 35(1):148-53.
Ingram, J.I., A. Antao-Menezes, E.A. Turpin, D.G. Wallace, J.B. Mangum, L.J. Pluta, R.S. Thomas, and J.C. Bonner . (2007) Genomic analysis of human lung fibroblasts exposed to vanadium pentoxide to identify candidate genes for occupational bronchitis. Respir. Res. 8:34.
Mangum, J.B., E.A. Turpin, A. Antao-Menezes, M.F. Cesta, E. Bermudez, and J.C. Bonner (2006) Single-walled carbon nanotube (SWCNT)-induced interstital fibrosis in the lungs of rats is associated with increased PDGF mRNA and the formation of unique carbon structures that bridge alveolar macrophages in situ. Particle & Fibre Toxicol. 3:15.
Ingram, J.I., A. Antao-Menezes, J.B. Mangum, O. Lyght, P.J. Lee, J.A. Elias, and J.C. Bonner. (2006) Opposing actions of Stat1 and Stat6 on IL-13-induced up-regulation of Egr-1 and PDGF ligands in pulmonary fibroblasts. J. Immunol. 177:4141-4148.
Walters, D.M. J.L. Ingram, A.A. Menezes, A. Nyska, Y. Tani, S.R. Kleeberger, and J.C. Bonner. (2005) Susceptibility of STAT-1-deficient mice to pulmonary fibrogenesis. Am. J. Pathol. 167: 1221-9.
Bonner, J. C. (2004). Regulation of PDGF and its receptors in fibrotic diseases. Cytokine Growth Factor Rev. 15, 255-273.