Research in the Planchart lab combines high throughput techniques, including proteomics and transcriptomics, to understand how environmental factors affect vertebrate embryonic development. We are focused on identifying genetic modifiers of craniofacial development that can be modulated by changes in the environment during embryogenesis. Specifically, we are interested in discovering regulatory genes that confer phenotypic plasticity, which buffer an organism from changes in its environment that could drastically alter its developmental program if left unchecked.
Our model organism is the zebrafish (Danio rerio). You might know it as the Zebra Danio, a small aquarium fish that is easy to keep at home and lives for 1 to 2 years, or more. The advantages of using zebrafish in biomedical research include:
- Evolutionary conservation: Zebrafish, like humans, are vertebrates. We shared a common ancenstor with zebrafish approximately 450 million years ago, compared to 800 million years with round worms (C. elegans) and fruit flies (D. melanogaster);
- Their genome is sequenced, which allows us to compare our genome with theirs in an attempt to identify conserved regulatory regions;
- Females release hundreds of eggs per spawning event, which are externally fertilized and easy to collect;
- Zebrafish embryos are transparent, which allows us to view in real-time things like cell movements, organ development, growth, etc.
For these and other reasons, zebrafish have become a powerful genetic and developmental model for understanding how we grow, how we interact with our environment, and how we become sick due to genetic deficiencies or harmful exposures.
Potential graduate students and post-doctoral fellows interested in our research are encouraged to contact Dr. Planchart directly. Graduate students interested in obtaining a Masters or Ph.D. are encouraged to apply to one of the following programs: Genetics, Toxicology, or Zoology.
- Bugel SM, Tanguay RL, Planchart A. Zebrafish: A marvel of high-throughput biology for 21st century toxicology. Curr Environ Health Rep. 2014 Sep 7;1(4):341-352. Abstract
- Cheng KC, Hinton DE, Mattingly CJ, Planchart A. Aquatic models, genomics and chemical risk management. Comp Biochem Physiol C Toxicol Pharmacol. 2012. 155:169-73. Abstract
- Planchart A, Mattingly CJ. 2,3,7,8-Tetrachlorodibenzo-p-dioxin upregulates FoxQ1b in zebrafish jaw primordium. Chem Res Toxicol. 2010. 23:480-7. Abstract
- Mattingly CJ, Hampton TH, Brothers KM, Griffin NE, Planchart A. Perturbation of defense pathways by low-dose arsenic exposure in zebrafish embryos. Environ Health Perspect. 2009. 117:981-7. Abstract
- Coffman JA, Coluccio A, Planchart A, Robertson AJ. Oral-aboral axis specification in the sea urchin embryo III. Role of mitochondrial redox signaling via H2O2. Dev Biol. 2009. 330:123-30. Abstract
- Planchart A. Analysis of an intronic promoter within Synj2. Biochem Biophys Res Commun. 2013. 440:640-5. Abstract
- Doukeris CE, Planchart A. Characterization of a Novel DNA Motif in the Tctex1 and TCP10 Gene Complexes and its Prevalence in the Mouse Genome. Adv Biol Res. 2007. 1:1-16. Abstract
- Schimenti JC, Reynolds JL, Planchart A. Mutations in Serac1 or Synj2 cause proximal t haplotype-mediated male mouse sterility but not transmission ratio distortion. Proc Natl Acad Sci U S A. 2005. 102:3342-7. Abstract
- Planchart A, Schimenti JC. Experimental and computational approaches yield a high-resolution, 1-Mb physical map of the region harboring the mouse t haplotype sterility factor, tcs1. Mamm Genome. 2001. 12:668-70. Abstract
- Browning VL, Chaudhry SS, Planchart A, Dixon MJ, Schimenti JC. Mutations of the mouse Twist and sy (fibrillin 2) genes induced by chemical mutagenesis of ES cells. Genomics. 2001. 73:291-8. Abstract
- Planchart A, You Y, Schimenti JC. Physical mapping of male fertility and meiotic drive quantitative trait loci in the mouse t complex using chromosome deficiencies. Genetics. 2000. 155:803-12. Abstract
- Schilling J, Nepomuceno AI, Planchart A, Yoder JA, Kelly RM, Muddiman DC, Daniels HV, Hiramatsu N, Reading BJ. Machine learning reveals sex-specific 17β-estradiol-responsive expression patterns in white perch (morone americana) plasma proteins. Proteomics. 2015 Apr 21. doi: 10.1002/pmic.201400606.[Epub ahead of print] Abstract
X-ray Fiber Diffraction:
- Wang H, Planchart A, Stubbs G. Caspar carboxylates: the structural basis of tobamovirus disassembly. Biophys J. 1998. 74:633-8. Abstract
- Wang H, Planchart A, Allen D, Pattanayek R, Stubbs G. Preliminary X-ray diffraction studies of ribgrass mosaic virus. J Mol Biol. 1993. 234:902-4. Abstract
- Schlax PE, Zhang J, Lewis E, Planchart A, Lawson TG. Degradation of the encephalomyocarditis virus and hepatitis A virus 3C proteases by the ubiquitin/26S proteasome system in vivo. Virology. 2007. 360:350-63. Abstract
Databases and Bioinformatics:
- Blake JA, Richardson JE, Bult CJ, Kadin JA, Eppig JT, et al. The Mouse Genome Database (MGD): the model organism database for the laboratory mouse. Nucleic Acids Res. 2002 30:113-5. Abstract
- Blake JA, Richardson JE, Bult CJ, Kadin JA, Eppig JT, et al. MGD: the Mouse Genome Database. Nucleic Acids Res. 2003 31:193-5. Abstract